Research Overview
This research project aims to develop an innovative enzymatic process for producing partially hydrolyzed galactomannan (PHGM) from Palmyra palm (Borassus flabellifer) endosperm using a thermostable mannanase. Palmyra palm is an underutilized tropical resource in Indonesia with significant potential as a sustainable source of functional hydrocolloids and prebiotic oligosaccharides. However, native galactomannan exhibits high viscosity and poor solubility, limiting its application in modern food systems.
The project addresses this challenge by applying thermostable enzymatic hydrolysis to tailor the molecular structure of galactomannan while preserving its functional properties. Unlike conventional acid hydrolysis, enzymatic hydrolysis provides greater selectivity, enabling the production of oligosaccharides with improved physicochemical characteristics and enhanced biological functionality. The use of thermostable enzymes also supports integration into industrial thermal processing, offering higher process efficiency and scalability.
The research focuses on optimizing enzyme concentration, pH, temperature, substrate concentration, and hydrolysis time to maximize the production of functional oligosaccharides while minimizing excessive monosaccharide formation. The resulting products will be characterized using advanced analytical techniques, including ATR-FTIR, Differential Scanning Calorimetry (DSC), optical microscopy, and scanning electron microscopy (SEM). Their prebiotic potential will be evaluated through the growth response of beneficial probiotic bacteria, particularly Lactobacillus plantarum.
This project represents an important step toward transforming Palmyra palm into a high-value ingredient for functional foods and nutraceuticals. It contributes to sustainable food innovation by promoting the utilization of indigenous plant resources, reducing dependence on imported hydrocolloids and prebiotic ingredients, and supporting Indonesia’s circular bioeconomy strategy. The research is also expected to establish a scalable platform for future industrial production of prebiotic oligosaccharides and the development of next-generation functional food products.