Makassar, Indonesia 22/06/2026/BioVax TB/ – On 22 October 2025, a laboratory activity entitled “PCR Gen rv3875–rv2873 and rv3874–rv2389c” was successfully conducted at the Laboratory of the Hasanuddin University Medical Research Center (HUMRC), Faculty of Medicine, Hasanuddin University (Lab HUMRC FK UNHAS). This activity focused on the polymerase chain reaction (PCR) amplification of the rv3875–rv2873 and rv3874–rv2389c gene regions to verify that the amplified DNA fragments corresponded to the expected template sizes. PCR remains one of the most reliable molecular biology techniques for confirming the presence and integrity of specific genetic targets before proceeding to more advanced analyses, such as sequencing, cloning, or functional characterization. Ensuring that amplified DNA fragments match the expected molecular size is a critical quality control step that strengthens the reliability and reproducibility of subsequent molecular research.
The primary objective of this laboratory work was to determine whether the DNA fragments generated from the rv3875–rv2873 and rv3874–rv2389c gene regions exhibited sizes consistent with the original templates. Accurate amplification and size verification are fundamental to molecular microbiology because they provide confidence that the target genetic regions have been successfully amplified without significant contamination or nonspecific products. During the experiment, standardized laboratory protocols were followed carefully, including DNA preparation, PCR reagent formulation, thermal cycling, and verification of PCR products through molecular analysis. Every stage of the workflow emphasized precision, consistency, and adherence to laboratory quality standards to ensure dependable experimental outcomes.
The activity was carried out by Nurul Husna and Noer Zakiah Derajat Sam, whose collaborative efforts contributed to the successful completion of the experiment. Beyond generating reliable laboratory data, this activity also provided valuable practical experience in molecular diagnostic techniques, laboratory quality assurance, and scientific problem-solving. Such hands-on experience enhances technical competence while reinforcing critical thinking, analytical skills, and the ability to conduct research using internationally recognized laboratory practices. Continuous participation in activities like this strengthens the capacity of young researchers to contribute meaningfully to scientific innovation and evidence-based healthcare.
This laboratory activity also supports several Sustainable Development Goals (SDGs). It directly contributes to SDG 3: Good Health and Well-being by improving the quality of molecular research that underpins disease diagnosis and biomedical innovation. It advances SDG 4: Quality Education by providing practical research training and strengthening scientific competencies among researchers and laboratory personnel. Furthermore, it promotes SDG 17: Partnerships for the Goals through collaborative research activities conducted within the research environment of HUMRC, encouraging knowledge sharing, scientific cooperation, and institutional capacity building. Collectively, these contributions demonstrate how fundamental laboratory research serves as an essential foundation for advancing medical science, strengthening research excellence, and supporting sustainable global health development.
